red fluorescent protein trap beads Search Results


anti dsred  (TaKaRa)
99
TaKaRa anti dsred
Anti Dsred, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m 2 r yfp  (Thermo Fisher)
86
Thermo Fisher m 2 r yfp
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
M 2 R Yfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m 2 r yfp - by Bioz Stars, 2026-02
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gfp lim expression constructs  (Thermo Fisher)
86
Thermo Fisher gfp lim expression constructs
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
Gfp Lim Expression Constructs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
gfp lim expression constructs - by Bioz Stars, 2026-02
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gfp e2  (TaKaRa)
86
TaKaRa gfp e2
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
Gfp E2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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active rhobenhanced gfp egfp  (TaKaRa)
86
TaKaRa active rhobenhanced gfp egfp
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
Active Rhobenhanced Gfp Egfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r1i81 yfp  (TaKaRa)
86
TaKaRa r1i81 yfp
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
R1i81 Yfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yfp-420apa-calm  (Agilent technologies)
90
Agilent technologies yfp-420apa-calm
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
Yfp 420apa Calm, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant marburg virus expressing the fluorescent zsgreen1  (Marburg GmbH)
90
Marburg GmbH recombinant marburg virus expressing the fluorescent zsgreen1
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
Recombinant Marburg Virus Expressing The Fluorescent Zsgreen1, supplied by Marburg GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant marburg virus expressing the fluorescent zsgreen1/product/Marburg GmbH
Average 90 stars, based on 1 article reviews
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nanog-mcherry fusion protein  (Carl Zeiss)
90
Carl Zeiss nanog-mcherry fusion protein
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
Nanog Mcherry Fusion Protein, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ires/hrgfp (humanized renilla green fluorescent protein) reporter cassette  (Agilent technologies)
90
Agilent technologies ires/hrgfp (humanized renilla green fluorescent protein) reporter cassette
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
Ires/Hrgfp (Humanized Renilla Green Fluorescent Protein) Reporter Cassette, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p ac-dsred-qf2  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare p ac-dsred-qf2
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
P Ac Dsred Qf2, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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serum against gfp  (Becton Dickinson)
90
Becton Dickinson serum against gfp
GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.
Serum Against Gfp, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.

Journal: PLoS ONE

Article Title: Gi/o-coupled muscarinic receptors co-localize with GIRK channel for efficient channel activation

doi: 10.1371/journal.pone.0204447

Figure Lengend Snippet: GIRK channel was activated by M 2 R-YFP (left) and MC9-YFP (right) but not by M 1 R-YFP (middle). (A) Schematic diagrams in upper panels depict the tested muscarinic receptors and GIRK1/2 channel. Shown in the middle panels are the current traces recorded in 140 mM KCl bath solution. Cells were held at -80 mV and the ramp pulse (-120 to 40 mV for 400 ms) was applied every 5 s. The black bars on the traces indicate the timing of agonist application. Basal and maximal current amplitude was measured at the holding potential before (a) and during (b, red) oxo-M application. (B) Expanded traces corresponding to “a” (black lines) and “b” (red lines) are shown in middle panels. The ramp pulse protocol is shown above the expanded trace. The agonist-induced current densities at a holding potential of -80 mV (ΔI max ) was measured. (C) Summary of ΔI max is shown as bars. Numbers of data are shown in parentheses. ***:p≤0.001, n.s.:p>0.05.

Article Snippet: HEK293T cells were transfected with the plasmid DNAs (μg for 4 x 10 4 cells) of M 1 R-YFP (0.8), MC9-YFP constructs (0.8), M 2 R-YFP (1.2), GIRK1/2 (0.7), GIRK1/2-CFP (1.2), Gα-FPs (1.1), Gα qi5 (1.0), Gβ 1 (0.8), Gγ 2 (0.5), M 1 R, MC9 or M 2 R tandem constructs (1.2), CFP-PH (1.1) or prestin-YFP (1.0) [ ] using LipofectAMINE 2000 (1 μL, Invitrogen, Carlsbad, CA, USA).

Techniques:

Inhibitory effects of PTX treatment on the GIRK channel activation by M 2 R-YFP tandem constructs.

Journal: PLoS ONE

Article Title: Gi/o-coupled muscarinic receptors co-localize with GIRK channel for efficient channel activation

doi: 10.1371/journal.pone.0204447

Figure Lengend Snippet: Inhibitory effects of PTX treatment on the GIRK channel activation by M 2 R-YFP tandem constructs.

Article Snippet: HEK293T cells were transfected with the plasmid DNAs (μg for 4 x 10 4 cells) of M 1 R-YFP (0.8), MC9-YFP constructs (0.8), M 2 R-YFP (1.2), GIRK1/2 (0.7), GIRK1/2-CFP (1.2), Gα-FPs (1.1), Gα qi5 (1.0), Gβ 1 (0.8), Gγ 2 (0.5), M 1 R, MC9 or M 2 R tandem constructs (1.2), CFP-PH (1.1) or prestin-YFP (1.0) [ ] using LipofectAMINE 2000 (1 μL, Invitrogen, Carlsbad, CA, USA).

Techniques: Activation Assay, Construct

(A) Amino acid sequences of i3 of mutant and chimeric constructs of MC9-YFP (black: M 1 R sequences, red: M 2 R sequences). (B) Summary of ΔI max . Circles represent agonist-induced GIRK channel current density (ΔI max ) upon application of oxo-M (10 μM). (C) Summary of FRET efficiency between MC9-YFP constructs and GIRK1/2-CFP in the absence of the agonist. FRET efficiency was calculated by acceptor bleaching (see experimental procedures). Fluorescent intensities of YFP and CFP in each combination under TIRF illumination were measured and are summarized in . (D) Correlation between ΔI max and FRET efficiency. ΔI max is plotted as a function of FRET efficiency for each MC9-YFP construct (open circles). (E) Activation of Gqi5 by MC9-YFP constructs. Schematic diagram of monitoring of the activation of Gqi5 is shown in the upper panel. Under TIRF illumination, application of oxo-M decreased the intensity of CFP tethered at the PH domain (I CFP-PH ), as shown in lower traces in the absence of PTX treatment (open circles). The inhibitory effects of PTX (filled circles) are summarized in . (F) Summary of agonist-induced decrease in I CFP-PH (ΔI CFP-PH ). Circles represent ΔI CFP-PH upon application of oxo-M (10 μM). Numbers of experiments are indicated in parentheses. *:0.01<p≤0.05, **:0.001<p≤ 0.01, n.s.:p>0.05.

Journal: PLoS ONE

Article Title: Gi/o-coupled muscarinic receptors co-localize with GIRK channel for efficient channel activation

doi: 10.1371/journal.pone.0204447

Figure Lengend Snippet: (A) Amino acid sequences of i3 of mutant and chimeric constructs of MC9-YFP (black: M 1 R sequences, red: M 2 R sequences). (B) Summary of ΔI max . Circles represent agonist-induced GIRK channel current density (ΔI max ) upon application of oxo-M (10 μM). (C) Summary of FRET efficiency between MC9-YFP constructs and GIRK1/2-CFP in the absence of the agonist. FRET efficiency was calculated by acceptor bleaching (see experimental procedures). Fluorescent intensities of YFP and CFP in each combination under TIRF illumination were measured and are summarized in . (D) Correlation between ΔI max and FRET efficiency. ΔI max is plotted as a function of FRET efficiency for each MC9-YFP construct (open circles). (E) Activation of Gqi5 by MC9-YFP constructs. Schematic diagram of monitoring of the activation of Gqi5 is shown in the upper panel. Under TIRF illumination, application of oxo-M decreased the intensity of CFP tethered at the PH domain (I CFP-PH ), as shown in lower traces in the absence of PTX treatment (open circles). The inhibitory effects of PTX (filled circles) are summarized in . (F) Summary of agonist-induced decrease in I CFP-PH (ΔI CFP-PH ). Circles represent ΔI CFP-PH upon application of oxo-M (10 μM). Numbers of experiments are indicated in parentheses. *:0.010.05.

Article Snippet: HEK293T cells were transfected with the plasmid DNAs (μg for 4 x 10 4 cells) of M 1 R-YFP (0.8), MC9-YFP constructs (0.8), M 2 R-YFP (1.2), GIRK1/2 (0.7), GIRK1/2-CFP (1.2), Gα-FPs (1.1), Gα qi5 (1.0), Gβ 1 (0.8), Gγ 2 (0.5), M 1 R, MC9 or M 2 R tandem constructs (1.2), CFP-PH (1.1) or prestin-YFP (1.0) [ ] using LipofectAMINE 2000 (1 μL, Invitrogen, Carlsbad, CA, USA).

Techniques: Mutagenesis, Construct, Activation Assay